Lipocube

Lipocube™ is a medical device company based in London, established to develop, design, patent, and market medical devices based on human fat tissue processing with applications primarily in regenerative medicine, as well as other medical fields like orthopaedics, aesthetics, urology and wound care.

Lipocube is already present in 93 countries around the world, has both CE and FDA approval for their devices and are renowned as leaders in regenerative medicine devices.

Mechanical SVF Isolation

BACKGROUND

Regenerative therapies using autologous stem cells are the emerging hot topic in medicine. Stem cell therapies are applied not only in aesthetics and dermatology, but also in orthopaedics wound healing, and in cardiovascular fields.

Harvesting regenerative cells, usually from bone marrow and culturing these in GMP standardised facilities, is a very long process taking weeks and is also very expensive. These factors hindered this field from growing widely for many years.

This changed dramatically after discovering these regenerative stem cells could be attained easily from fat. More precisely, stromal vascular fraction (SVF) that is derived from adipose tissue has become a reliable and effective source, containing abundant numbers of regenerative cells. Fat tissue has 500-1000 times more regenerative cells in comparison to bone marrow and is in a concentrated form.

Regarded as the gold standard in terms of cell count in SVF, enzymatic digestion of fat is still relatively expensive by nature, as well as being time consuming (75-90 minutes). These factors have limited the usage of fat derived stem cells.

Lipocube™ SVF

Autologous fat is harvested from patient without the need of general anaesthesia. The fat tissue is mechanically disaggregated using the Lipocube™ in a closed sterile system with patented specific structural geometric blades coupled with a flow pattern. The disaggregated fat tissue is further incubated by mixing it with the Lipocube™ buffer solution, enhancing separation of regenerative cells from fat tissue by molecular electrical charges. The resulting emulsion is processed in the specially designed Lipocube™ device named Cell-Drive™ within a predefined RCF spectrum, and the SVF is concentrated in a patented trap with a specific cell attracting coating and geometry. The SVF is then applied to the patient.

Mechanical methods of SVF isolation deliver lower cell yields per cc of lipoaspirate processed in comparison to enzymatic methods; however, the composition of the cell populations in the mechanically extracted SVF are generally similar to enzymatic digestion.

The Lipocube™ is a state-of-the-art mechanical digestion device for the isolation of SVF from lipoaspirate.

ADVANTAGES OF LIPOCUBE™ SVF

• There is no need for a GMP facility / Your practice is basically your lab.

• It is an easy to use device enabling practicians high cell yields.

• Isolation time is shorter.

• There is no need for expert staff.

• The manipulation of cells is minimum, a key point in legal issues.

• It is a cost-efficient method.

• Fully enclosed system thus sterile environment.